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. 2014 May 9;14:328. doi: 10.1186/1471-2407-14-328

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Copyright © 2014 Jung et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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YB-1 binds to the SOX2 promoter and regulates Sox2 transcripts. (A) Schematic diagram of the Sox2 proximal promoter (2.5 kb upstream of transcriptional start site, denoted by +1) with markings of the minimal YB-1 consensus sequence ATTG/CAAT. Sox2 promoter ChIP primers designed against putative binding sites are shown. (B) MCF7 and ZR751 RU and RR ChIP DNA agarose gel results of DNA sequences immunoprecipitated by normal rabbit IgG or a rabbit anti-human YB-1 antibody amplified by Sox2 promoter specific primers a and d. MCF7 Input and ZR751 Input represent the DNA isolated from chromatin before immunoprecipitation to show equal input amounts. (C) Quantitative-RT-PCR results illustrating Sox2 mRNA transcript levels after 72-hour 20nM YB-1 siRNA treatment. Western blot shows YB-1 knockdown efficiency.