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. 2014 May;4(Suppl 1):S298–S304. doi: 10.12980/APJTB.4.2014B341

Table 1. Values of different biochemical parameters of control and VSSA treated group.

Parameters 1h
2h
3h
6h
12h
24h
Control Treated Control Treated Control Treated Control Treated Control Treated Control Treated
Superoxide anion generation [(nmol/(min · 106 cells)] 46.871±1.057 70.745±0.997 48.698±1.167 92.458±0.942* 47.872±0.985 128.437±0.853* 50.412±1.48 117.399±0.796* 47.345±0.956 93.991±0.979* 45.315±1.118 79.265±0.859*
NADPH oxidase activity [(nmol/(min · 106 cells)] 46.871±1.057 68.745±0.997* 48.698±1.167 88.458±0.942* 47.872±0.985 108.437±0.853* 50.412±1.48 97.399±0.796* 47.345±0.956 93.991±0.979* 45.315±1.118 83.265±0.859*
NO generation (µmol/mg protein) 23.13±1.975 40.953±1.605* 24.302±1.663 59.443±2.910* 23.769±1.662 117.045±2.186* 25.493±1.261 96.567±1.246* 22.663±1.115 82.3175±1.451* 23.667±0.989 69.301±0.972*
MPO activity (µmol/mg protein) 17.13±1.975 35.953±1.605* 17.302±1.663 49.443±2.910* 17.769±1.662 67.045±2.186* 17.493±1.261 61.567±1.246* 17.663±1.115 55.317±1.451* 17.667±0.989 49.301±0.972*
MDA level (µmol/mg protein) 27.13±1.975 35.953±1.605* 27.302±1.663 49.443±2.910* 27.769±1.662 69.045±2.186* 27.493±1.261 60.567±1.246* 27.663±1.115 54.375±1.451* 27.667±0.989 49.301±0.972*
PC level (µmol/mg protein) 17.13±1.975 31.953±1.605* 17.302±1.663 39.443±1.910* 17.769±1.662 44.567±1.186* 17.493±1.261 42.375±1.246* 17.663±1.115 40.301±1.451* 17.667±0.989 38.012±0.972*
GSH level (µg of GSH/mg protein) 1.415±0.045 0.885±0.055* 1.463±0.018 0.677±0.023* 1.385±0.045 0.308±0.029* 1.395±0.035 0.455±0.065* 1.352±0.035 0.595±0.065* 1.401±0.027 0.701±0.053*
GSSG level (µg of GSSG/mg protein) 0.685±0.055 1.015±0.055* 0.677±0.018 1.063±0.023* 0.678±0.045 1.185±0.029* 0.675±0.035 1.095±0.065* 0.675±0.035 0.992±0.065* 0.671±0.027 0.941±0.053*
SOD activity (IU/mg protein) 33.679±0.955 29.664±0.891 33.014±0.865 17.105±0.862* 32.541±0.925 12.569±0.957* 33.472±0.954 14.593±0.903* 31.052±0.942 17.362±0.875* 30.256±0.842 19.321±0.972*
CAT activity (µmol H2O2 consumed/(min · mg) protein) 16.325±0.286 13.256±0.312 16.236±0.214 10.062±0.232* 16.324±0.274 8.012±0.189* 16.239±0.189 8.735±0.261* 16.148±0.342 9.365±0.232* 16.023±0.232 11.002±0.341*
GPx activity (nmol NADPH consumed/min · mg protein) 6.325±0.086 4.256±0.092* 6.236±0.084 4.062±0.092* 6.324±0.074 3.212±0.089* 6.239±0.089 3.335±0.061* 6.148±0.082 3.765±0.092* 6.023±0.082 4.002±0.091*
GR activity (nmol NADPH consumed/min · mg protein) 9.325±0.216 7.256±0.312* 9.236±0.214 6.062±0.232* 9.324±0.214 4.612±0.189* 9.239±0.189 4.835±0.261* 9.148±0.242 5.765±0.232* 9.023±0.232 6.002±0.341*
GST activity (nmol NADPH consumed/min · mg protein) 12.325±0.286 7.256±0.312* 12.236±0.214 6.062±0.232* 2.324±0.274 3.612±0.189* 12.239±0.189 4.835±0.261* 12.148±0.342 6.765±0.232* 12.023±0.232 7.002±0.341*

Values are expressed as mean±SEM, n=6. *Significant difference (P<0.05) in VSSA treated group compared to control group at respective time interval.