Table 1. Values of different biochemical parameters of control and VSSA treated group.
Parameters | 1h |
2h |
3h |
6h |
12h |
24h |
||||||
Control | Treated | Control | Treated | Control | Treated | Control | Treated | Control | Treated | Control | Treated | |
Superoxide anion generation [(nmol/(min · 106 cells)] | 46.871±1.057 | 70.745±0.997 | 48.698±1.167 | 92.458±0.942* | 47.872±0.985 | 128.437±0.853* | 50.412±1.48 | 117.399±0.796* | 47.345±0.956 | 93.991±0.979* | 45.315±1.118 | 79.265±0.859* |
NADPH oxidase activity [(nmol/(min · 106 cells)] | 46.871±1.057 | 68.745±0.997* | 48.698±1.167 | 88.458±0.942* | 47.872±0.985 | 108.437±0.853* | 50.412±1.48 | 97.399±0.796* | 47.345±0.956 | 93.991±0.979* | 45.315±1.118 | 83.265±0.859* |
NO generation (µmol/mg protein) | 23.13±1.975 | 40.953±1.605* | 24.302±1.663 | 59.443±2.910* | 23.769±1.662 | 117.045±2.186* | 25.493±1.261 | 96.567±1.246* | 22.663±1.115 | 82.3175±1.451* | 23.667±0.989 | 69.301±0.972* |
MPO activity (µmol/mg protein) | 17.13±1.975 | 35.953±1.605* | 17.302±1.663 | 49.443±2.910* | 17.769±1.662 | 67.045±2.186* | 17.493±1.261 | 61.567±1.246* | 17.663±1.115 | 55.317±1.451* | 17.667±0.989 | 49.301±0.972* |
MDA level (µmol/mg protein) | 27.13±1.975 | 35.953±1.605* | 27.302±1.663 | 49.443±2.910* | 27.769±1.662 | 69.045±2.186* | 27.493±1.261 | 60.567±1.246* | 27.663±1.115 | 54.375±1.451* | 27.667±0.989 | 49.301±0.972* |
PC level (µmol/mg protein) | 17.13±1.975 | 31.953±1.605* | 17.302±1.663 | 39.443±1.910* | 17.769±1.662 | 44.567±1.186* | 17.493±1.261 | 42.375±1.246* | 17.663±1.115 | 40.301±1.451* | 17.667±0.989 | 38.012±0.972* |
GSH level (µg of GSH/mg protein) | 1.415±0.045 | 0.885±0.055* | 1.463±0.018 | 0.677±0.023* | 1.385±0.045 | 0.308±0.029* | 1.395±0.035 | 0.455±0.065* | 1.352±0.035 | 0.595±0.065* | 1.401±0.027 | 0.701±0.053* |
GSSG level (µg of GSSG/mg protein) | 0.685±0.055 | 1.015±0.055* | 0.677±0.018 | 1.063±0.023* | 0.678±0.045 | 1.185±0.029* | 0.675±0.035 | 1.095±0.065* | 0.675±0.035 | 0.992±0.065* | 0.671±0.027 | 0.941±0.053* |
SOD activity (IU/mg protein) | 33.679±0.955 | 29.664±0.891 | 33.014±0.865 | 17.105±0.862* | 32.541±0.925 | 12.569±0.957* | 33.472±0.954 | 14.593±0.903* | 31.052±0.942 | 17.362±0.875* | 30.256±0.842 | 19.321±0.972* |
CAT activity (µmol H2O2 consumed/(min · mg) protein) | 16.325±0.286 | 13.256±0.312 | 16.236±0.214 | 10.062±0.232* | 16.324±0.274 | 8.012±0.189* | 16.239±0.189 | 8.735±0.261* | 16.148±0.342 | 9.365±0.232* | 16.023±0.232 | 11.002±0.341* |
GPx activity (nmol NADPH consumed/min · mg protein) | 6.325±0.086 | 4.256±0.092* | 6.236±0.084 | 4.062±0.092* | 6.324±0.074 | 3.212±0.089* | 6.239±0.089 | 3.335±0.061* | 6.148±0.082 | 3.765±0.092* | 6.023±0.082 | 4.002±0.091* |
GR activity (nmol NADPH consumed/min · mg protein) | 9.325±0.216 | 7.256±0.312* | 9.236±0.214 | 6.062±0.232* | 9.324±0.214 | 4.612±0.189* | 9.239±0.189 | 4.835±0.261* | 9.148±0.242 | 5.765±0.232* | 9.023±0.232 | 6.002±0.341* |
GST activity (nmol NADPH consumed/min · mg protein) | 12.325±0.286 | 7.256±0.312* | 12.236±0.214 | 6.062±0.232* | 2.324±0.274 | 3.612±0.189* | 12.239±0.189 | 4.835±0.261* | 12.148±0.342 | 6.765±0.232* | 12.023±0.232 | 7.002±0.341* |
Values are expressed as mean±SEM, n=6. *Significant difference (P<0.05) in VSSA treated group compared to control group at respective time interval.