Figure 2.

ubc-25 is a negative regulator of intestinal cell cycles. (A) Schematic diagram of the ubc-25 locus. Exons and introns are indicated by boxes and connecting lines, respectively. Gray shading indicates sequences encoding the E2 domain. Location of catalytic cysteine is labeled “C.” Region deleted by ok1732 mutation is delineated by labeled line. (B) Image of wild-type L2-aged animal with intestinal nuclei highlighted by the elt-2::gfp transgene within rtIs14. (C) Image of typical ubc-25(ok1732) age-matched animal displaying extra intestinal nuclei. Scale bars indicate 20 μm. (D) Quantification of intestinal nuclei of the indicated genotypes and ages. ztEx223 is an extrachromosomal array containing the Pubc-25::mCherry::UBC-25 expression plasmid. The median, 25%, and 75% quartiles are shown as centerline and lower and upper box edges, respectively. The whiskers indicate the total range of values (n ≥ 15). (E) Comparison of representative cell lineages observed for the intestinal E lineages of (left) wild type (wt; n = 2 embryos) and (right) ubc-25(ok1732) (n = 5 embryos) animals. The wild-type lineage is indistinguishable from lineages previously observed in wild type (Yan et al. 2013).