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. 2014 Feb 28;4(5):795–804. doi: 10.1534/g3.114.010546

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Copyright © 2014 Roy et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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ubc-25 is a negative regulator of cye-1. (A) Box and whisker plot presenting the effect of ubc-25(RNAi) on intestinal nuclei number in self-progeny of cye-1(+/−); rtIs14 hermaphrodites. For each cye-1 experimental pair, connected by horizontal lines, the RNAi treatment of either ubc-25 or the negative control unc-73 is indicated above. n ≥ 15 for each condition. (B) Western blot illustrating increased expression of CYE-1 upon inhibition of ubc-25 activity by RNAi. Steady-state expression of CYE-1 is increased at least threefold (n = 3). TBA-1/α-tubulin is used as a loading and normalization control.