Table 1. Enhanced cell-cycle quiescence defect of ubc-25(lf); lin-35(lf) animals.
Genotype | RNAia | % Elm Animalsb | n |
---|---|---|---|
Wild type | unc-73 | 0 | 65 |
Wild type | lin-35 | 0 | 90 |
Wild type | ubc-25 | 2 | 46 |
lin-35(n745) | unc-73 | 0 | 27 |
lin-35(n745) | ubc-25 | 23 | 26 |
ubc-25(ok1732) | unc-73 | 6 | 109 |
ubc-25(ok1732) | lin-35 | 22 | 96 |
RNAi, RNA interference; VPC, vulval precursor cell.
unc-73(RNAi) is used as the negative control.
The Elm cell-cycle quiescence defects were scored using the more sensitive measure of extra VPC production by directly examining animals at the L2-to-L3 molt.