Figure 5.

Odor stimulation rapidly affects 3 mRNAs. (A) Filtered air chambers for controlling odor exposure. Room air is pumped through 2 activated carbon filters, split into 2 streams, hydrated, directed past tubes containing stimuli and then down over individual mice sitting on a wire mesh floor in a conical chamber designed to allow wastes and odors to be carried away to an exhaust stream captured by a fume hood. Capacity is 8 mice: 4 chambers for each of the 2 odor streams. Stimulation with odor or vehicle is done via electronically controlled pinch valves (large circles) that redirect the air stream through a tube containing either odor or vehicle. Small ovals: flow meters. (B) Initial experiment testing S100A5 and Lrrc3b (n = 4). (C) Experiment testing 10 additional mRNAs (n = 8). (B and C) horizontal line: mean value; solid rectangle: 1 standard deviation; vertical bars: range of data; (*): significant difference. This figure is reproduced in color in the online version of the issue.