Fig. 4.
Scatter plot analysis of the high throughput LOPAC pilot screen. (A) All data from all assay plates (n=12, triplicate results), including controls, are displayed. The separation in activity (Z′=0.57±0.06, S/B=1.7±0.1) between wells dosed with IN3 (“high control”) and DMSO (“low control”) indicates a HTS assay that will allow for reproducible selection of hits. (B) Representative graph of IN3 pharmacoperone activity performed while running the LOPAC pilot screen. The EC50 (5.4 nM) indicates reproducible and expected assay sensitivity was achieved. The data presented are means±sd of quadruplicate wells (n=4).