Skip to main content
. 2014 May 2;53(18):2926–2940. doi: 10.1021/bi500002n

Figure 8.

Figure 8

Simulation of iron-containing species in WT(W303) (black), WT(DY150) (blue), Δccc1 (red), CCC1-up (green), and CCC1-up plus adenine (pink) cells at different concentration of FC in minimal medium. Solid lines are simulations for a particular strain/condition. Circles are data with the same color-coding. The dashed lines indicate the threshold concentration for cytosolic Fe. Parameters used in all simulations: kin = 16 h–1; km = 4 h–1; α = 0.5 h–1; Kc = 21 μM; nc =5; Km1 = 24 μM; nm1 = 3; Km2 = 90 μM; mn2 = 3; Kv1 = 17 μM; nv1= 6; Kv2 = 200 μM; nv2 = 4. Parameters specific for the following strains, including kccc1 (h–1), k23 (h–1), K32 (μM), n32, and knp (h–1): WT(W303), 38, 16, 18, 9, and 0.04; WT(DY150), 38, 13, 18, 9, and 0.2; Δccc1, 4, 1, 16, 9, and 0.15; CCC1-up, 400, 1, 12, 3, and 2; CCC1-up + adenine, 400, 9, 16, 9, and 0.3. Specific simulation and data values, along with residuals, are given in Table S3.