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. 2014 Jun 1;20(16):2479–2496. doi: 10.1089/ars.2012.4794

FIG. 1.

FIG. 1.

Changes in protein carbonylation and Grp94 levels in unloaded soleus muscles. Dots indicate mobility of molecular weight markers (Broad Range; BioRad Lab., Rome, Italy). n indicates the number of muscles evaluated in each group. *Student's t-test p=0.05; **p=0.01. (A) Unloading increases the degree of muscle protein carbonylation determined by Oxyblot assay, as described in “Materials and Methods”. Left side: representative RP and Oxyblot (Oxy) stainings of a same blot from a 10% acrylamide denaturing gel. Right side: bars and error bars correspond to mean and SE of densitometric values of protein carbonylation normalized to SA of ambulatory (A) and unloaded (U) muscles analyzed in parallel. (B) Unloading decreases specifically muscle levels of Grp94. Left side: Representative western blot for Grp94, Grp78, Grp75, and Csq from a 10% acrylamide denaturing gel loaded with 50 μg of whole muscle homogenates. Reference for sample loading is shown by RP staining for SA and immunolabeling for GAPDH. Right side: bars and error bars correspond to mean and SE of normalized densitometric values of samples analyzed in parallel. Csq, calsequestrin; GAPDH, glyceraldehyde-3-phosphate deydrogenase; RP, red ponceau; SA, serum albumin; SE, standard error.