Skip to main content
. 2014 Jun 1;20(16):2606–2620. doi: 10.1089/ars.2013.5406

FIG. 6.

FIG. 6.

Ubiquinol-10 increased mitochondrial metabolic activity by promoting deacetylation of PGC-1α by SIRT1. (A) HepG2 cells were treated with the indicated ubiquinol-10 doses for 48 h. The cell lysates (10 μg) were analyzed by western blot. HepG2 cells were treated with 0.5, 1, 3, and 10 μM ubiquinol-10. (B) Immunoblot analysis of PGC-1α deacetylation in HepG2 cells after treatment with or without 3 μM ubiquinol-10 in the presence of the SIRT1 inhibitor nicotinamide (20 mM). (C) Molecular interactions of SIRT1 and PGC-1α were determined after treatment with or without ubiquinol-10 in the presence of nicotinamide. (D) Phosphorylation of AMPK and AMPK substrate ACC was determined after treatment with or without ubiquinol-10 (3 μM) in the presence of the AC inhibitor MDL-12,330A (50 μM). (E) Oxygen consumption was measured in HepG2 cells with or without ubiquinol-10 (n=10). (F) Oxygen consumption rate (rate of fluorescence intensity/min) is shown in the panel (n=10). (G) ELISA analysis to determine NAD and NADH levels in HepG2 cells with or without ubiquinol-10 (n=6). *p<0.05; **p<0.01. AC, adenylate cyclase; ACC, acetyl-CoA carboxylase.