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Compound 7l inhibited C9 deposition through the classical, the lectin, and the alternative pathways but had no influence on C3 and C4 depositions. Normal human serum (NHS) was added to IgM-coated plates (classical pathway), mannan-coated plates (lectin pathway), or zymosan-coated plates (alternative pathway) and then incubated with various concentrations of compound 7l. Deposited C3 (A), C4 (B), and C9 (C) were measured by ELISA.
