. 2012 May 17;3(6):445–449. doi: 10.1021/ml200241a

Table 1. IC₅₀ values for compounds in the CDK enzyme assays^a,^b.

graphic file with name ml-2011-00241a_0004.jpg

			IC₅₀ (μM)
	X₁	X₂	CDK4/Cyclin D1	CDK6/Cyclin D3	CDK1/Cyclin B	CDK2/Cyclin A
2	N	CH	0.006 ± 0.001	0.049 ± 0.006	1.2 ± 0.2	0.27 ± 0.06
3	N	N	0.012 ± 0.002	0.3 ± 0.1	>15	6.2 ± 0.4
4	CCN	N	0.003 ± 0.000	0.026 ± 0.003	5.6 ± 0.4	2.4 ± 0.2
5	N	CH	0.25 ± 0.03	NT	>15	>15
6	CCN	N	0.015 ± 0.001	0.12	>15	>15

Results are expressed as the mean ± standard deviation of 1–2 IC₅₀ determinations. For each determination, concentration–inhibition curves were obtained in duplicate and then averaged to afford a single IC₅₀ curve with a ≥95% confidence interval.

70% homology shared between CDK4 and -6 contributes to comparable SAR profiles of two enzymes.

Table 1. IC50 values for compounds in the CDK enzyme assaysa,b.

Table 1. IC₅₀ values for compounds in the CDK enzyme assays^a,^b.