Figure 4.
SD1002 preserves synaptic markers in APPSwInd mice. The 20-month transgenic mice were injected ip daily with SD1002 (+) or vehicle (−) for 9 days, and wild-type mice (wt) were subjected to vehicle injections. (a) Equal protein aliquots of hippocampal homogenates were analyzed by standard SDS-PAGE and immunoblotting for GluR1 and amphiphysin I (Amph I) as well as a load control. (b) Mean GluR1 immunoreactivity levels ± SEMs are shown for the three treatment groups. Unpaired t test as compared to vehicle-treated transgenic group: **P < 0.001. (c) Representative hippocampal samples were also separated on tris-tricine gels to assess oligomeric Aβ species and the parent hAPP recognized by 6E10 antibody and a load control in the same blot lanes. Standard gel protocol assessed for synapsin II (syn II) and the active form of cathepsin B (act CB) in corresponding samples.