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Four examples of the frequently observed spacing between the contrast enhanced vessel lumens of the hepatic artery and portal vein, observed by preparation method 1. For vessels < 0.5 mm in diameter, the hepatic artery and portal vein lumens remain constantly spaced at ∼ 40 μm apart (represented by dashed white lines). This spacing is likely the result of the concomitant vessel walls touching (as the vessel walls are not enhanced by contrast).
