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Localization of CENP-A by immunofluorescence. a CENP-A staining (red) in interphase nucleus, foci represent centromeres. b CENP-A staining (red) of metaphase spreads. CENP-A localizes to the kinetochore of each sister chromatid. All imaging performed in nuclei isolated from a lymphoblast cell line at 100X magnification. DNA is stained with DAPI (blue)
