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Brilliant Yellow and Direct Violet 51 are potent inhibitors of vesicular glutamate uptake. Rat brain synaptic vesicles were pre-incubated in the presence of various concentrations of Brilliant Yellow (left) or Direct Violet 51 (right) in a mixture described in Materials and Methods, followed by addition of a mixture of [3H]glutamate ± ATP, and an additional 10-min incubation.
