Table 3.

Effect of functional group alterations of Brilliant Yellow and 4,4’-dinitrostilbene-2,2’-disulfonate on vesicular glutamate uptake inhibition by Brilliant Yellow

Test compounds	IC50 μM
Brilliant Yellow	0.044 ± 0.006a
Chrysophenine	0.266 ± 0.070b
4,4’-Dinitrostilbene-2,2’-disulfonate	1.69 ± 0.11c
4,4’-Dibenzamidostilbene-2,2’-disulfonate	2.0d
4,4-Diacetamidostilbene-2,2’-disulfonate	> 50
4,4-Diphenylsulfonamidostilbene-2,2’-disulfonate	> 50
4,4-Diaminostilbene-2,2’-disulfonate	> 50
4,4-Di-p-hydroxybenzylidene-aminostilbene-2,2’-disulfonate	> 50
4,4-Dinitrostilbene-2,2’-sulfonic acid diphenyl ester	> 50

Synaptic vesicles were incubated with [³H]glutamate in the presence of various concentrations of test compounds, as described in Fig. 1.

^aTaken from Table 1;

^bmean ± SEM (n=4);

^cmean ± SEM (n=3;

^dDetermined from Fig. 4.