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. Author manuscript; available in PMC: 2014 Jul 23.
Published in final edited form as: Mol Cell. 2013 Dec 26;53(2):290–300. doi: 10.1016/j.molcel.2013.11.012

Figure 1. Identification of the RNA-binding region of JARID2.

Figure 1

(A) Domain organization of human JARID2 and scheme of the 6xHis-fused truncations utilized in the mapping experiments.

(B) In vitro streptavidin pull-down after incubation of increasing concentrations of the indicated JARID2 recombinant fragments with biotinylated HOTAIR1–333. Input, 2 μg; titration, 2 and 4 μg.

(C) High resolution mapping of the residues of JARID2 necessary for RNA binding in vitro. The two indicated fragments (right) were incubated with HOTAIR1–333 and assayed as in (B). Input, 2 μg; titration, 1, 2, and 4 μg.

See also Figure S1.