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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Biomaterials. 2014 Feb 28;35(15):4454–4464. doi: 10.1016/j.biomaterials.2014.02.001

Fig. 3.

Fig. 3

Calcium transient analysis of hESC-CMs seeded onto lanes of varying widths. Live fluorescent imaging was conducted, and intensities were measured for ROIs at opposite ends of each lane (A). By comparing normalized calcium transient intensity over time (B) between the two ROIs, and using the distance between the ROIs, the calcium propagation rate was determined for each micropattern width. ROI=region of interest, FN=fibronectin.