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. Author manuscript; available in PMC: 2014 May 19.
Published in final edited form as: Virology. 2013 Jun 25;444(0):124–139. doi: 10.1016/j.virol.2013.05.042

Figure 2. MMTV acquired via the natural route regulates BST-2 expression.

Figure 2

Age-matched C3H/HeN•MMTV- and C3H/HeN•MMTV+ mice (n = 3) were bred. Their pups (n = 5/time point) were sacrificed at different time points (3, 7, 21, and 30 days) as shown on the figures. At necropsy, splenocytes were subjected to FACS analysis of BST-2 surface protein (A and B), RT-qPCR of BST-2 mRNA expression (C), and PCR of MMTV proviral DNA levels (D). PBMCs were obtained from adult (5 weeks old) C3H/HeN•MMTV− (naïve) and C3H/HeN•MMTV+ (infected) mice (n = 3) prior to sacrifice. At necropsy, spleens and lymph nodes, as well as the isolated PBMCs were subjected to RT-qPCR examination of BST-2 levels following RNA isolation and cDNA synthesis (E), FACS analysis of surface BST-2 (F). DNA isolated from the cells was used for PCR examination of proviral DNA (G). RT-PCR data are normalized to GAPDH and presented as relative levels (C) or as fold change relative to BST-2 mRNA in naïve mice (E). GAPDH was also used as loading control (D and G). Error bars are standard deviation; ** is significance with p value less than 0.01 and * is significance with p value less than 0.05. The numbers next to histogram legend denote mean florescence intensity (MFI) of surface BST-2 levels. Experiments were repeated at least three times with similar results.