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. Author manuscript; available in PMC: 2014 May 19.
Published in final edited form as: Virology. 2013 Jun 25;444(0):124–139. doi: 10.1016/j.virol.2013.05.042

Figure 6. Mammary carcinoma epithelial cells overexpress BST-2 and viral nuclei acids.

Figure 6

Normal mammary epithelial cells (NMuMG) and carcinoma mammary epithelial cells (4T1 and Mm5MT) were used for RNA extraction and RT-qPCR examination of BST-2 mRNA (A). A portion of cells were used for FACS analysis of BST-2 surface protein (B). RNA isolated from mammary epithelial cells (NMuMG and Mm5MT), mammary gland from tumor-bearing mice (n = 5) and mammary tumor tissues from the same mice were reverse transcribed and used to examine BST-2 transcripts by RT-qPCR (C). DNA, RNA, and protein isolated from cells and tissues as indicated in the figure were examined for levels of proviral DNA and viral RNA (D) as well as viral protein (E). RT-qPCR and qPCR data are normalized to GAPDH and presented as fold change relative to samples from NMuMG or Mm5MT. Viral protein was detected with MMTV capsid antibody (α- p27) and GAPDH was used as loading control. Error bars are standard deviation; ** is significance with p value less than 0.01 and * is significance with p value less than 0.05. The numbers next to histogram legend denote mean florescence intensity (MFI) of surface BST-2 levels. All experiments were repeated 3 times with similar results.