Figure 7. Expression levels of some PI3K isoforms in C3H/HeN mammary gland and tumor tissues positively correlate with BST-2.
RNA isolated from mammary gland and tumor tissues from Naïve mice (n = 5), infected mice (n = 5), and tumor-bearing mice (n = 5) described in figure 5, or mammary epithelial cells (NMuMG and Mm5MT) described in figure 6 was used. Transcript levels of PI3KCA, PI3KCB, PI3KCD, and PI3KCG in mammary gland tissues (A) and mammary gland and tumor tissues (B) were compared by RT-qPCR analysis. Level of PI3KR1 mRNA was compared between mammary gland tissues and tumor tissue (C). PI3KCA, PI3KCB, PI3KCD, PI3KCG, and PI3KR1 mRNAs in mammary epithelial cells were analyzed by RT-qPCR (D and E). Graphs represent means and error bars are standard deviation; ** is significance with p value less than 0.01 and * is significance with p value less than 0.05. Experiments were repeated 3 separate times with similar results.
