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. Author manuscript; available in PMC: 2014 May 19.
Published in final edited form as: Virology. 2013 Jun 25;444(0):124–139. doi: 10.1016/j.virol.2013.05.042

Figure 8. AKT1, AKT3, and MIP1α/CCL3 mRNA correlate with BST-2 but AKT2 mRNA does not correlate with BST-2 in mammary gland and tumor tissues of C3H/HeN.

Figure 8

Transcript levels of AKT1, AKT2, and AKT3 in mammary gland, tumor tissues and mammary epithelial cells were determined by RT-qPCR analysis of cDNA obtained from total RNA of naïve mice (n = 5), infected mice (n = 5), mice bearing mammary tumor (n = 5), NMuMG, and Mm5MT cells (A to C). AKT protein level was determined by Western blot using total cell lysate probed with phospho-specific antibodies and total AKT antibody for loading control (D and E). Expression level of MIP1α/CCL3 in mammary gland, tumor tissues and mammary epithelial cells were determined by RT-qPCR analysis of cDNA obtained from total RNA of naïve (n = 5), infected (n = 5), mice bearing mammary tumor (n = 5), NMuMG, and Mm5MT cells (F and G). Graphs represent means and error bars are standard deviation; ** is significance with p value less than 0.01 and * is significance with p value less than 0.05. Experiments were repeated 3 separate times with similar results.