Figure 3. Autophagy and apoptotic responses in H4 cells during starvation. (A) Images of GFP-LC3 and the corresponding segmentation results after serum deprivation for indicated periods of time. (B) Distributions of autophagosome scores after serum deprivation. (C) Images of H2B-RFP and the corresponding segmentation results after serum deprivation for indicated periods of time. (D) Distributions of death scores after serum starvation. (E) Images of GFP-LC3 and the corresponding segmentation results after glucose deprivation for indicated periods of time. (F) Distributions of autophagosome scores after glucose deprivation. (G) Images of H2B-RFP and the corresponding segmentation results after glucose deprivation for indicated periods of time. (H) Distributions of death scores after glucose starvation. To make the height of the curve insensitive to the size of bins used, the total area under each density function curve was normalized to 1.