(A) HIV Gag-specific CD8+ T-cell responses in PBMC (PB) and
rectal mucosa (RM) pre- and post-HAART, showing individual factors (IL-2,
TNF-α, IFN-γ, CD107) produced in response to Gag peptide
stimulation. Pale gray boxes indicate pre-HAART; dark boxes indicate post-HAART
values. (B) Total Gag-specific CD8+ T-cell response in PBMC (PB)
and rectal mucosa (RM), pre- and post-HAART, when all non-overlapping responses
are combined. P values were determined by Wilcoxon test. (C)
Polyfunctionality of CD8+ T-cell responses to HIV Gag peptides. The percentage
value shown in the center of each pie diagram denotes the total percentage of
CD8+ T-cells responding in any way to Gag peptide stimulation. Individual
color-coded pie slices represent the fraction of cells within the total
responding population displaying 4 (white), 3 (red), 2 (yellow), or 1 (blue)
function(s), beginning at the top of each pie and moving clock-wise, as compiled
from the bar graph data. The bar graph provides fine detail on frequencies
within each of the individual 15 categories. Individual bars are color-coded in
bright green (PBMC, pre-HAART), avocado (PBMC, post-HAART), dark blue (Rectal
Mucosa, pre-HAART), or aqua (Rectal Mucosa, post-HAART). Bar heights show
interquartile ranges (25%–75%), with horizontal black
lines indicating median values.