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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Cell Microbiol. 2013 Jan 7;15(6):942–960. doi: 10.1111/cmi.12088

Fig. 3. Constitutive production of flagellin does not impair replication of B. melitensis 16M in macrophages in vitro, but attenuates its virulence in vivo.

Fig. 3

(A) Western blot analysis of flagellin (FliC, upper panel) production in wt and BruFliCON strains during early exponential and stationary phases of growth in 2YT rich medium. Detection of Omp89 was used as a loading control. (B) Intracellular replication of wt and BruFliCON strains in RAW264.7 murine macrophages. Error bars represent the standard deviation of triplicates in one representative experiment out of two. (C) Infection kinetics in the spleens of wt BALB/c mice (n=5) inoculated i.p. with 4 × 104 CFUs of wt or BruFliCON strain. Data represent the mean CFUs per organ and error bars represent standard deviation. Results have been analyzed by ANOVA I after testing the homogeneity of variance (Bartlett). ** and *** denote highly significant (p<0.01 and p<0.001 respectively) differences in relation to wt infection. These results are representative of at least two independent experiments.