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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Cell Microbiol. 2013 Jan 7;15(6):942–960. doi: 10.1111/cmi.12088

Fig. 5. B. abortus flagellin induces IL-1β in an NLRC4-independent manner.

Fig. 5

(A) Primary bone marrow-derived macrophages from C57BL/6 mice were primed with LPS and inoculated with B. melitensis 16M wt or the ΔfliC mutant and IL-1β was measured in the culture supernatants by ELISA. Results are shown as the mean ± standard deviation of data from an individual experiment that was repeated 4 times with similar results. (B) Immortalized, LPS-primed C57BL/6 or Nlrc4-/- bone marrow-derived macrophages were inoculated with B. melitensis 16M wt or the BruFliCONstrain. IL-1β in the supernatant was measured at 6h after inoculation. Data shown are combined from three independent experiments with triplicate samples, and represent the mean ± standard deviation of all data.