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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Cell Microbiol. 2013 Jan 7;15(6):942–960. doi: 10.1111/cmi.12088

Fig. 6. Introduction of recombinant Brucella flagellin into the host cell cytosol results in ASC-dependent, but NLRC4-independent secretion of IL-1β.

Fig. 6

Graded amounts of GST-BruFliC and GST-StFliC fusion proteins were delivered to the cytosol of LPS-primed primary bone marrow-derived macrophages from C57BL/6 (A), Nlrc4-/- (B) or Asc-/- (C) mice, using the cationic lipid DOTAP. Treated macrophages were incubated for 3h before measurement of IL-1β in the supernatants by ELISA. Results are expressed as the mean of triplicate samples, with error bars representing the range of the data from one of two independent experiments with the same outcome.