Table 1.
Bacterial strains and plasmids used in this study.
| Designation | Genotype and/or Phenotype | Source or Reference |
|---|---|---|
| Strains | ||
| Brucella melitensis strains | ||
| 16M | wild type isolate | |
| Δ fliC | ΔfliC::Kan | (Ferooz et al., 2011) |
| Δ flbT | ΔflbT::Kan | (Ferooz et al., 2011) |
| Δ fliF | ΔfliF::Kan | (Ferooz et al., 2011) |
| BruFliCON | pBBR1-fliC | This work |
| Brucella abortus strain | ||
| 2308 | wild type isolate | |
| Salmonella enterica serovar Typhimurium strains | ||
| 14028 | ATCC 14028 Wild-Type | ATCC |
| IR715 | 14028 Spontaneous NalR | (Stojiljkovic et al., 1995) |
| LT2 | LT2 Wild-Type | (Lilleengen, 1948) |
| EHW26 | IR715 fliC::Tn10 fljB::MudJ (fliCfljB) | (Raffatellu et al., 2005) |
| Escherichia coli strains | ||
| CC118 λpir | araD139 Δ(ara, leu)7697 ΔlacX74 phoAΔ20 galE galK thi rpsE rpoB argEam recA1 λpir | (Simon et al., 1983) |
| DH10B | F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara, leu)7697 galU galK rpsL(StrR) endA1 nupG | Invitrogen |
| DH5 | F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacΔZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 phoA supE44 λ- thi-1 gyrA96 relA1 | (Woodcock et al., 1989) |
| S17-1 λpir | recA thi pro rK– mK+ RP4:2-Tc:MuKm Tn7 λpir | (Simon et al., 1983) |
| Plasmids | ||
| pCR2.1 | TOPO cloning vector | Invitrogen |
| pUC-KIXX | pUC4::Tn5 KanR | (Beck et al., 1982) |
| pBBR1MCS | mob RK2, lacZα, CmR | (Kovach et al., 1994) |
| pRH001 fliC | pMR10 (CmR, B. melitensis 16M PfliC-fliC-tfliC) | This work |
| pBBR1-fliC | pBBR1MCS(CmR, B. melitensis 16M fliC) | This work |
| pBBRFlag | pBBR1MCS::3xFLAG | This work |
| pYHS1116 | pBBRFlag::StFliC | This work |
| pYHS1073 | pBBRFlag::BaFliC | This work |
| pWSK29 | CarbR, pSC101 ori | (Wang et al., 1991) |