. Author manuscript; available in PMC: 2014 Jun 1.

Published in final edited form as: Cell Microbiol. 2013 Jan 7;15(6):942–960. doi: 10.1111/cmi.12088

Table 2.

Primers used in this work

Primer	Sequence	Restriction site	Application
BaFliC-F	ACCATATGGCTAGCATTCTTACAAACTCGTCG	NdeI	FLAG-tagged BaFliC and BaFliC::FT fusion protein
BaFliC-R	ACTGCAGTTAGCCGCGGAACAGCGACAGGATCGAC	SalI	FLAG-tagged BaFliC and BaFliC::FT fusion protein
StFliC-F	ACCATATGGCACAAGTCATTAATACAAACAGC	NdeI	FLAG-tagged StFliC and StFliC::FT fusion protein
StFliC-R	AGTCGACTTAACGCAGTAAAGAGAGGACGTTTTGC	SalI	FLAG-tagged StFliC and StFliC::FT fusion protein
StFliC-F2	*GAATTC*ATGGCACAAGTCATTAATACAAACAGC	EcoRI	GST-StFliC fusion protein
StFliC-STOP-R	*ACTCGA*GTTAACGCAGTAAAGAGAGGACGTTTTGC	Xhol	GST-StFliC fusion protein
BaFliC-F2	*GAATTC*ATGGCTAGCATTCTTACAAACTCG	EcoRI	GST fusion proteins
BaFliC-R2	*ACTCGAG*TTAGCCGCGGAACAGCGACAG	Xhol	GST fusion proteins
PfliC	CGGGATCCAATGCCCGGGATCATGTTGATGC	BamHI	complementation plasmid
tfliC	GCTCTAGATGCCAGACAGGATGTCGGGC	XbaI	complementation plasmid
Plac	GCTCtagAtagAtagAGCGCAACGCAATTAATGTGAG	XbaI	fliC overexpression plasmid
fliC-Plac	GTTTGTAAGAATGCTAGCCATAGCTGTTTCCTGTGTGAAATTG
BmfliC-F	ATGGCTAGCATTCTTACAAACTCGT
BmfliC-R	CGGGATCCTTAGCCGCGGAACAGCG	BamHI

Bold: Extra 5’ DNA; Bold/Underlined: Multiple cloning site; Bold/Underlined/Italicized: Restriction site utilized in cloning; Lower case: Start or stop codon.