Figure 2. FIH-1 is one of the miR-31 targets and is negatively regulated by miR-31. (A) FIH-1 expression in HCT116 or SW1116 cells after transfection with anti-miR-31 siRNA or miR-31 mimics detected by western blotting. (B) FIH-1 expression was negatively correlated with miR-31 in 3 pairs of CRC tumor and peritumor tissues. (C) Predicted consequential pairing of target 3′UTR region of FIH-1 (wild-type or mutated) and miR-31 mature sequence. Luciferase activity on the presence of both wild-type FIH-1 3′UTR or mutant and miR-31 was compared with the control.