Figure 3. Effect of miR-31/FIH-1 nexus on proliferation, migration, invasion of HCT116 and SW1116 cells. (A) The transfection efficiency of anti-miR-31 siRNA and the promotion of FIH-1 expression in HCT116 and SW1116 cells. (B) Inhibition of miR-31 (promotion of FIH-1 expression) had significant effect on decreasing proliferation rate of both cell lines. (C) Wound closure was delayed in cells transfected with anti-miR-31 siRNA as compared with negative control in 24 h time points in both types of CRC cells. (D) Representative fields of invasion (down) or migration (up) cells on the membrane was on the left (magnification of 200×). Average invasion or migration cell number per field was on the right. The invasion or migration cell number of HCT116 and SW1116 transfected with anti-miR-31 siRNA was drastically decreased than that transfected with pairing negative control. **P < 0.01, ***P < 0.001.