Figure 5. Knockdown of CD95 or FADD protects cells from pazopanib and valproate treatment. HT1080 cells were transfected with scrambled control siRNA (siSCR) or siRNA molecules to knockdown expression of CD95 (siCD95) or FADD (siFADD). Thirty-six hours after transfection cells were treated with vehicle (DMSO), pazopanib (P, 5.0 μM), and/or valproate (V, 500 μM). Cells were isolated 24 h after exposure and viability determined by trypan blue exclusion (n = 3, ± SEM). ^#P < 0.05 less than corresponding value in siSCR cells.