Figure 1. (A) Relative fold change in PLD1 mRNA and Chk-α mRNA in patient-derived tumor samples that are either ER⁺ (n = 11) or ER⁻ (n = 8). (B) Immunoblots representing the protein expression of Chk-α in nonmalignant MCF-12A cells, non-metastatic ER⁺ MCF-7 cells and highly metastatic ER⁻ MDA-MB-231 cells. (C) Immunoblots representing the protein expression of PLD1 in MCF-12A, MCF-7, and MDA-MB-231 cells. GAPDH was used as a loading control.