Figure 7. (A) Representative high-resolution 1H MR spectra showing the GPC, PC, Cho region of MDA-MB-231 cell extracts: untreated (1), treated with 25 nM PLD1-siRNA (2), treated with 100 nM PLD1-siRNA (3), treated with 25 nM PLD1-siRNA + 75nM Chk-α-siRNA (4), treated with 75 nM Chk-α-siRNA (5), and treated with100 nM Chk-α-siRNA (6). (B) Histogram showing quantified levels of PC (white box), GPC (gray box), and tCho (black box), in MDA-MB-231 cells (n = 4 per group): untreated (1), treated with Dfect (2), treated with 100 nM control siRNA (3), treated with 25 nM PLD1-siRNA (4), treated with 100 nM PLD1-siRNA (5), treated with 75 nM Chk-α-siRNA (6), treated with 100 nM Chk-α-siRNA (7), and treated with 25 nM PLD1-siRNA + 75 nM Chk-α-siRNA (8). Values represent mean + SE, *P ≤ 0.05, compared with untreated control cells unless otherwise indicated.