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. 2014 May 13;13:80. doi: 10.1186/1476-511X-13-80

Table 1.

Methods and primers used in genotyping of TAFI −438 A/G, +1583 A/T, −1690 A/G and −2345 2G/1G polymorphisms

Gene polymorphisms Analysis Primers
TAFI −438 A/G
PCR-RFLP
Forward:ACCAATGGGAATGTAGG
Reverse:CCTTGCCTCTGACTTTT
TAFI +1583 A/T
PCR-RFLP
Forward:CTTGGCATGTCATTAGG
Reverse:TGCGGCATTTGTTGACA
TAFI −1690 A/G
AS-PCR
Common Forward a:CACCTGTAGACTTTTGC
Special Forward a1:TTAACTATTTTGACTGTTTA
Special Forward a2:TTAACTATTTTGACTGTTTG
Common Reverse b: CACTGAAGGAGAGAAAG
TAFI −2345 2G/1G AS-PCR Common Forward a:CACCTCAACTGGACTATGT
Special Forward a1:AGTTTTTAAAACATGAAAGA
Special Forward a2:AGTTTTTAAAACATGAAAGGA
Common Reverse b: TGTTCCCTTGCAGTTTAGC