Figure 1.
SIRT1 is expressed in adult NSCs and neural progenitors. (a) SIRT1 is expressed in the nuclei of NESTIN-positive cells in adult NSC and neural progenitor cultures. Immunocytochemistry on adherent monolayer cultures of adult NSCs and neural progenitors using antibodies against SIRT1 (green) and against the NSC/progenitor marker NESTIN (red). DAPI (blue) is a nuclear marker. (b) SIRT1 is expressed in NSCs and neural progenitors in the SVZ of the adult brain. Immunohistochemistry on brain sections of 8-week-old mice using antibodies against SIRT1 (green) and SOX2 (red, upper panel) or Ki67 (red, lower panel). White arrowheads show examples of double-stained cells. Insets show magnification of a cell positive for SIRT1 and SOX2 or Ki67. Merged images include DAPI to illustrate nuclei. Scale bars, 50 μm.