Figure 5.

SIRT1 inactivation enhances remyelination in the corpus callosum after lysolecithin-induced demyelination. Mice lacking active SIRT1 in the nervous system exhibit enhanced remyelination after focal lysolecithin-induced demyelination in the corpus callosum (CC). Sirt1^lox/lox and NestinCre;Sirt1^lox/lox 8-week-old mice were stereotactically injected with 1% lysolecithin in the corpus callosum and allowed to recover for 5 or 14 days. Levels of myelination were blindly quantified in the region of injection by coverage of MBP staining over background. One-tailed, unpaired Student’s t-test, *P = 0.028. Mean±s.e.m. of n = 3 Sirt1^lox/lox and n = 5 NestinCre;Sirt1^lox/lox mice for day 5 and n = 10 Sirt1^lox/lox and n = 8 NestinCre;Sirt1^lox/lox mice for day 14. Arrowheads indicate demyelination areas. Scale bars, 50 μm.