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SMase C inhibition of Kv1.3. a, b, Ionic currents of wild-type Kv1.3 (a) and gating currents of the W384F mutant (b) elicited at 5 second intervals with the voltage protocol shown, gradually declining following addition of recombinant SaSMase C (the off-gating current is largely immobilized). c, d, G–V (c) and Qon-V (d) relations before (squares) and after (dots) treatment with SaSMase C. The data are presented as mean ± s.e.m. (n = 9–11).
