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. 2014 May 19;9(5):e97326. doi: 10.1371/journal.pone.0097326

Table 7. Table of primers used for quantitative RT-PCR (qRT-PCR).

Primers for gene expression by RT-qPCR
Gene Forward Reverse
Esp 5'-GACTCTCAGAAGATTCACAGTTGC-3' 5'-AAAGCCCAGGCTCAGGTT-3'
Phex 5'-TGCCAGAGAACAAGTGCAAA-3' 5'-CTAATGGCACCATTGACCCTA-3'
Vegf 5'-AAAAACGAAAGCGCAAGAAA-3' 5'-TTTCTCCGCTCTGAACAAGG-3'
Dmp1 5'-GGTTTTGACCTTGTGGGAAA-3' 5'-TTGGGATGCGATTCCTCTAC-3'
FGF23 5'-ATCTCCACGGCAACATTTTT-3' 5'-GTCCACTGGCGGAACTTG-3'
Plin2 5'-CTCCACTCCACTGTCCACCT-3' 5'-GCTTATCCTGAGCACCCTGA-3'
Bglap (osteocalcin) 5'-AGACTCCGGCGCTACCTT-3' 5'-CTCGTCACAAGCAGGGTTAAG-3'
Gapdh 5'-TGTCAAGCTCATTTCCTGGTATGA-3' 5'-CTTACTCCTTGGAGGCCATGTAG-3'
Sost (Sclerostin) 5'-TCCTGAGAACAACCAGACCA-3' 5'-GCAGCTGTACTCGGACACATC-3'
Mepe 5'-GATGCAGGCTGTGTCTGTTG-3' 5'-TGTCTTCATTCGGCATTGG-3
Cyclophilin 5'-CAGACGCCACTGTCGCTTT-3' 5'-TGTCTTTGGAACTTTGTCTGCAA-3'
24-hydroxylase 5'-AACTGTACGCTGCTGTCACG-3' 5'-AATCCACATCAAGCTGTTTGC-3'
1 α-hydroxylase 5'-AGTGGGGAATGTGACAGAGC-3' 5'-GGAGAGCGTATTGGATACCG-3'
NPT2c 5'-CAGCCCTGCAGACATGTTAAT-3 5'-GCACCAGGTACCACAGCAG-3'
NPT2a 5-GATTTGGTGTCACCCAGACA-3' 5'-ATGGCCTCTACCCTGGACAT-3'
Transferrin 5'-GACTCCGAACAACCTGAAGC-3' 5'-GCGTAGTAGTAGGTCTGTGGATGTT-3'
FAM20C 5'-TGAACAGCGACATCAGGTTT-3' 5'-CGCCTTCAGCACCTTCAT-3'
ENPP1 5'-CGGACGCTATGATTCCTTAGA-3' 5'-AGCACAATGAAGAAGTGAGTCG-3'