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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: DNA Repair (Amst). 2014 Feb 25;16:35–43. doi: 10.1016/j.dnarep.2014.01.002

Figure 3. A,B. Depletion of RSC complex in STH1-degron strain results in major deficiency of BER genome- wide.

Figure 3

The STH1-degron and WT strains were grown and MMS treated as described in materials and methods. To analyse NMPs removal genomic DNA was processed as described above. A. A representative gel showing repair time course in WT and STH1- depleted STH1-degron strains. M: marker DNA size standard (λ/HindIII); 0: cells damaged with MMS; 1–3: repair time course in hours. Positions of the approximate median migration distance of the fragments in each lane are shown with asterisks (*) and are connected by a dotted line. B. Repair time course of NMPs. Data represent mean ± 1 s.d. from three independent experiments.