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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Oncogene. 2013 May 27;33(18):2385–2394. doi: 10.1038/onc.2013.182

Figure 2. CA-RSK phosphorylates all of the putative RSK sites in hCdc25 isoforms.

Figure 2

Immobilized GST-hCdc25 proteins were mock-treated or phosphorylated with CA-RSK. Proteins eluted from washed beads were immunoblotted with each of the indicated phospho-specific antibodies after Ponceau S (Ponc) staining. The sequence context of the phosphorylation site recognized by each of the phospho-specific antibodies is indicated below immunoblots. (A–C) Phosphorylation of the wild type (WT) and S293A-S295A mutant form (2A) of GST-Cdc25A. (D&E) Phosphorylation of the wild type (WT) and S353A-T355V mutant form (AV) of GST-Cdc25B. (F) Phosphorylation of the wild type (WT) and S247A mutant form (1A) of GST-Cdc25C.