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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Oncogene. 2013 May 27;33(18):2385–2394. doi: 10.1038/onc.2013.182

Figure 5. RSK inhibitors partially inhibit phosphorylation of the RSK sites in hCdc25A and hCdc25B in mitotic cell lysates.

Figure 5

The immobilized GST-hCdc25A (A&B) and GST-hCdc25B (C&D) were phosphorylated with 1:30-diluted ME from HEK293 cells in the presence or absence of indicated concentrations of BI-D1870 (A&C) or SL0101 (B&D) at room temperature for 30 min. Proteins eluted from washed beads were immunoblotted with indicated phospho-specific antibodies.