Fig 3.

Tcf7l2 associates with nuclear beta-catenin and with Olig2. a Tcf7l2 (red) and beta-catenin (green) double staining in the mouse corpus callosum after 5 weeks of cuprizone treatment. Arrows point to the cells that are positive for Tcf7l2 and also exhibit weak nuclear beta-catenin expression. b The quantitative analysis of nuclear beta-catenin levels in Tcf7l2 positive cells. Comparing to the average of the highest nuclear beta-catenin levels in the same section, Tcf7l2-positive cells only associate with moderate levels of nuclear betacatenin (<40%) during the remyelination process. n, the total number of cells measured in each brain section. The error bar shows the value of the standard deviation. c Tcf7l2 protein can form protein complex with Olig2, but not with Olig1, in 293T cells. Cells are only transfected with Olig2-myc plasmid (Lane 1); only Tcf7l2-V5 plasmid (Lane 2); both Olig2-myc and Tcf7l2-V5 plasmids (Lane 3); both Olig2-myc and Tcf7l2-V5 plasmids with no antibodies used to pull down myc or V5 antigens (Lane 4, control); both Olig1-myc and Tcf7l2-V5 plasmids (Lane 5); both Olig1-myc and Tcf7l2-V5 plasmids with myc or V5 immunoblotting only (Lane 6). Arrowhead points to the Olig2 protein in the immunoblotting. Arrows indicate the positions of Tcf7l2, Olig1, and Olig2 proteins in the immunoblotting determined by molecular weight. d Tcf7l2 (red) is not expressed with Ki67 (green) in the mouse corpus callosum after 5 week cuprizone treatment