FIG. 5.

Altering intra-molecular structure of ss-siRNAs to improve allele-selective inhibition of HTT expression. (A) List of ss-siRNAs. Subscript “_s” indicates PS linkage; 2′-O-Me modified base is shown in uppercase; 2′-F in underlined uppercase; 2′-MOE in enclosed box. Mismatched base is shown in bold and italic. The terminal T has a 5′ phosphate. All other linkages are phosphate. (B) Effects of ss-siRNAs tested at 25 nM. (C) Effect of increasing concentration of ISIS 641384 and ISIS 641385, representative data from triplicate experiments. CM: non complementary duplex RNA.