Table 1. Primer sequences used for PCR products intended to generate In Situ Hybridization Probes (1) or for Real-Time Polymerase Chain Reaction (2).
Gene name | Orientation | Sequence (5′-3′) | Product length (bp) | Accession number |
CaMK1a (1) | ForwardReverse | AAGCACCCCAACATTGTAGC-AAGGCCTGCTCACAGGTAAA- | 595 | NM_133926 |
CaMK1a (2) | ForwardReverse | TGGCTACCCACCCTTTTATG-CTTGATCTGCTCGCTCACTG- | 247 | NM_133926 |
Atf3 (1) | ForwardReverse | CCACCCCACCTATCAAGGTA-GCTCAGAATGGACGGACAC- | 425 | NM_007498.3 |
Atf3 (2) | ForwardReverse | ACAACAGACCCCTGGAGATG-CCTTCAGCTCAGCATTCACA- | 187 | NM_007498.3 |
Sprr1a (2) | ForwardReverse | CCAGCAGAAGACAAAGCAGA-GGGCAATGTTAAGAGGCTCA- | 215 | NM_009264.2 |
Npy (2) | ForwardReverse | TGGACTGACCCTCGCTCTAT-TGTCTCAGGGCTGGATCTCT- | 187 | NM_023456.2 |
Polr2j (2) | ForwardReverse | ACCACACTCTGGGGAACATC-CTCGCTGATGAGGTCTGTGA- | 176 | NM_011293.2 |
Ddx48 (2) | ForwardReverse | GGAGTTAGCGGTGCAGATTC-AGCATCTTGATAGCCCGTGT- | 205 | NM_138669.1 |