Figure 5. SR-A1 knockout suppresses macrophage adhesion and migration induced by ER stress.

Peritoneal macrophages were isolated from WT and SR-A1^−/− mice after normal saline injection and cultured in 25(OH)D₃-treated media with or without Thapsigargin. A. Adhesion assay showing absorbance of cells adhered to fibronectin (n=4 per group, p<0.001 by ANOVA, *p<0.05 vs. all other groups by Tukey’s post-test). B. Transwell migration assay (n=4 per group, p<0.001 by ANOVA, *p<0.05 vs. all other groups by Tukey’s post-test, **p<0.05 between indicated groups by Tukey’s post-test). C–E. qPCR for mRNA of adhesion receptors C. β1-integrin and D. β2-integrin and E. migration receptor CCR2 (n=3 per group, p<0.005 by ANOVA for each, *p<0.05 vs. all other groups by Tukey’s post-test).