Skip to main content
. 2014 May 19;9(5):e96549. doi: 10.1371/journal.pone.0096549

Figure 2. Processing of colicin E2 in membrane-protein deficient strains.

Figure 2

The proteins of the crude cell extracts (CE) of wild type (lane 3, wt) TolA-deficient (lane 1, ΔTolA), porins OmpF and OmpC-deficient (lane 2, ΔOmpF-C), BtuB receptor-deficient (lane 4, ΔBtuB) and TolB-deficient (lane 5, ΔTolB), FtsH protease-deficient (lane 7, ΔFtsH) and carrying a plasmid-born wt ftsH gene (lane 6, pftsH) and OmpT-deficient (lanes 8, 9, ΔOmpT) strains grown in LB medium and treated with ColE2-ImmE2, were loaded onto 15% SDS-PAGE and analyzed by immunodetection with anti-colicin E2 antiserum.