Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 May 19;9(5):e96549. doi: 10.1371/journal.pone.0096549

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Mora, de Zamaroczy

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Analysis of the in vivo observed forms derived from the hybrid-colicins. The proteins of the crude cell extracts of an ompT-inactivated strain (AD202; ΔompT), treated by colicin E3 (lanes 1, 5, E3), colicin E2 (lanes 4, 8, E2), hybrid colicin E2/E3 (lanes 2, 6) or hybrid colicin E3/E2 (lanes 3, 7), were separated on 15% SDS-PAGE and analysed in parallel by Western blotting with anti-colicin E2 antiserum (left panel) or anti-colicin E3 antiserum (right panel). (B) Colicin forms detected in vivo from mutated colicins, were analysed as described in (A). ColE2 PF was quantified and expressed as a percentage of PF value (%), measured with colicin E2 (lane 3, 100%).