Table 1. Bacterial strains and plasmids used in this study.
Strain or plasmid | Genetic description | References |
AD202 | ompT1000::kan, KmR | [48] |
BL21(DE3) | ΔompT | [49] |
JCL8789 | 188 nadA::Tn10, ΔtolB-pal, TetR | [50] |
JCL11650 | 169 btuB::Tn10, TetR | [50] |
JCL11619 | 188 nadA::Tn10, ΔtolA, TetR | [50] |
JW2203-1 (9781) | ΔompC768::kan, KmR | CGSCa |
JCL8928 | 8056 zcb::Tn10, ΔompF627, TetR | [50] |
LM2013 | ΔompC768-ΔompF627, TetR, KmR | This work |
(by P1 transduction from JW2203-1 to JCL8928) | ||
AR3291/1734 | MG1655 sfhC zad::Tn10, ftsH3::kan, TetR, KmR | [10], [51] |
p ftsH | pACYC184, ftsH (at SalI/HinDIII), CmR | [10] |
pColE2-P9 | colicin E2 cea-cei-cel b | [52] |
pColE2I | pACDuet-1, cea-cei(tag-His6), (at NdeI/BamHI), CmR | This work |
pK317 | colicin E7 cea-cei-cel b | [53] |
pColE7I | pET11a, cea-cei(tag-His6), (at NdeI/BamHI), AmpR | This work |
pColE3I | pET11a, cea-cei(tag-His6) (at NdeI/BamHI), AmpR | [10] |
pColE3/ColE2-ImmE2 | hybrid colicin: junction at ColE3 R454/ColE2 K457 | This work |
pColE2/ColE3-ImmE3 | hybrid colicin: junction at ColE2 G456/ColE3 K455 | This work |
The Coli Genetic Stock Center.
cea, cei, cel are structural genes for colicin E encoding toxin, immunity and lysis proteins.